Question 1

One of the first uses of DNA fingerprinting was in _________ medicine.

Accepted Answer

The answer of One of the first uses of DNA...

Question 2

Which of the following methods determines which genes are actively transcribed in a cell under a variety of conditions?

Accepted Answer

A)  DNA fingerprinting
B)  microarray analysis
C)  gene mapping
D)  Western blot
E)  antisense therapyF) A) and E)
G) D) and E)

Question 3

All of the following are characteristics that make plasmids good cloning vectors, except

Accepted Answer

A)  they are small.
B)  they are well characterized.
C)  they can be transferred into appropriate host cells.
D)  they can carry genetic markers, such as antibiotic resistance genes.
E)  they can accept a relatively large amount of foreign DNA.F) A) and B)
G) C) and D)

Question 4

Restriction endonucleases recognize and clip at DNA base sequences called _____.

Accepted Answer

The answer of Restriction endonucleases recognize and clip at DNA...

Question 5

The commercial product Frostban contains a genetically engineered strain of

Accepted Answer

A)  Escherichia coli.
B)  Saccharomyces cerevisiae.
C)  Thermus aquaticus.
D)  Pseudomonas syringae.
E)  Pseudomonas fluorescens.F) B) and E)
G) None of the above

Question 6

The process of introducing a needed, normal gene, into human cells is called DNA mapping.

Accepted Answer

A) True 
 B)False

Question 7

Restriction endonucleases are obtained from various species of bacteria.

Accepted Answer

A) True 
 B)False

Question 8

All of the following are correct about DNA fingerprinting, except

Accepted Answer

A)  it displays the unique pattern of an individual's DNA fragments.
B)  it can be used to analyze evidence when only minute amounts of DNA are found.
C)  it cannot be used on specimens older than five years.
D)  it is routine to use it as trial evidence in the U.S.
E)  it can be used on hair and saliva.F) A) and B)
G) All of the above

Question 9

Vectors often contain a gene conferring drug resistance to their cloning host, in order to detect cells harboring the plasmid.

Accepted Answer

A) True 
 B)False

Question 10

When determining the sequence of nucleotides in an unknown sample of DNA, which method is used to sequence the DNA?

Accepted Answer

A)  PCR
B)  Cloning
C)  Sanger
D)  Southern blot hybridizationE) A) and B)
F) All of the above

Question 11

DNA polymerases used in PCR

Accepted Answer

A)  use an RNA template to make complementary DNA.
B)  must remain active at very cold temperatures.
C)  include Taq polymerases and Vent polymerase.
D)  are labeled with fluorescent dyes.
E)  All of the choices are correct.F) B) and E)
G) C) and D)

Question 12

Which technique will hopefully be used to identify and devise treatments for diseases based on the genetic profile of the disease?

Accepted Answer

A)  PCR
B)  genetic engineering
C)  antisense technology
D)  microarray analysis
E)  DNA sequencingF) A) and D)
G) A) and C)

Question 13

Reverse transcriptase is used to make cDNA from an RNA template.

Accepted Answer

A) True 
 B)False

Question 14

The Southern Blot technique detects

Accepted Answer

A)  DNA.
B)  RNA.
C)  proteins.
D)  recombinant DNA.
E)  specific genetic marker sequence on genes.F) B) and D)
G) B) and C)

Question 15

Thermococcus litoralis and Thermus aquaticus are thermophilic bacteria that are

Accepted Answer

A)  used as cloning vectors.
B)  sources of heat stable DNA polymerases.
C)  genetically engineered bacteria.
D)  principal sources of restriction endonucleases.
E)  None of the choices are correct.F) A) and C)
G) A) and D)

Question 16

Which of the following drugs is/are produced by genetic engineering and approved for human use?

Accepted Answer

A)  human growth hormone
B)  Factor VIII
C)  insulin
D)  tissue plasminogen activator
E)  All of the choices are correct.F) A) and E)
G) C) and D)

Question 17

Genomic _____ are collections of isolated genes maintained in a cloning host.

Accepted Answer

The answer of Genomic _____ are collections of isolated genes...

Question 18

The fluorescent in situ hybridization (FISH)  probes are applied to intact cells and observed microscopically for the presence and location of

Accepted Answer

A)  DNA.
B)  RNA.
C)  proteins.
D)  recombinant DNA.
E)  specific genetic marker sequence on genes.F) B) and D)
G) All of the above

Question 19

After 3 replication cycles in PCR, there will be a total of 4 double-stranded DNA molecules.

Accepted Answer

A) True 
 B)False

Question 20

The various techniques by which scientists manipulate DNA in the lab are called

Accepted Answer

A)  genetic engineering.
B)  biotechnology.
C)  recombinant DNA.
D)  gel electrophoresis.
E)  gene probes.F) B) and E)
G) A) and D)

Exam 10: Genetic Engineering: a Revolution in Molecular Biology

The various techniques by which scientists manipulate DNA in the lab are called

Correct AnswerverifiedShow Answer

Restriction endonucleases recognize and clip at DNA base sequences called _____.

Correct AnswerverifiedShow Answer

The process of introducing a needed, normal gene, into human cells is called DNA mapping.

Correct AnswerverifiedShow Answer

After 3 replication cycles in PCR, there will be a total of 4 double-stranded DNA molecules.

Correct AnswerverifiedShow Answer

The fluorescent in situ hybridization (FISH) probes are applied to intact cells and observed microscopically for the presence and location of

Correct AnswerverifiedShow Answer

One of the first uses of DNA fingerprinting was in _________ medicine.

Correct AnswerverifiedShow Answer

Genomic _____ are collections of isolated genes maintained in a cloning host.

Correct AnswerverifiedShow Answer

Which technique will hopefully be used to identify and devise treatments for diseases based on the genetic profile of the disease?

Correct AnswerverifiedShow Answer

The commercial product Frostban contains a genetically engineered strain of

Correct AnswerverifiedShow Answer

Reverse transcriptase is used to make cDNA from an RNA template.

Correct AnswerverifiedShow Answer

Vectors often contain a gene conferring drug resistance to their cloning host, in order to detect cells harboring the plasmid.

Correct AnswerverifiedShow Answer

Which of the following drugs is/are produced by genetic engineering and approved for human use?

Correct AnswerverifiedShow Answer

Thermococcus litoralis and Thermus aquaticus are thermophilic bacteria that are

Correct AnswerverifiedShow Answer

Which of the following methods determines which genes are actively transcribed in a cell under a variety of conditions?

Correct AnswerverifiedShow Answer

All of the following are correct about DNA fingerprinting, except

Correct AnswerverifiedShow Answer

The Southern Blot technique detects

Correct AnswerverifiedShow Answer

Restriction endonucleases are obtained from various species of bacteria.

Correct AnswerverifiedShow Answer

All of the following are characteristics that make plasmids good cloning vectors, except

Correct AnswerverifiedShow Answer

When determining the sequence of nucleotides in an unknown sample of DNA, which method is used to sequence the DNA?

Correct AnswerverifiedShow Answer

DNA polymerases used in PCR

Correct AnswerverifiedShow Answer

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