Question 1

Sequencing a gene automatically identifies which gene it is.

Accepted Answer

A) True 
 B)False

Question 2

The process of introducing a needed normal gene into human cells is called DNA mapping.

Accepted Answer

A) True 
 B)False

Question 3

Which PCR step causes the denaturation of double-stranded DNA?

Accepted Answer

A)  Add DNA polymerase and nucleotides at 72°C.
B)  Cool DNA to between 50°C and 65°C.
C)  Add primers.
D)  Heat target DNA to 94°C.
E)  Repeat the cycle of heating and cooling.F) A) and E)
G) A) and C)

Question 4

An example of gene therapy is the insertion of the gene for human growth hormone into E.coli cells.

Accepted Answer

A) True 
 B)False

Question 5

The function of the dideoxy (dd)  nucleotides that are used in the Sanger method of DNA sequencing is to

Accepted Answer

A)  denature DNA into single strands.
B)  serve as primers.
C)  be a fluorescent tag.
D)  incorporate into newly replicated DNA strands and stop elongation.E) None of the above
F) B) and C)

Question 6

Sequences of DNA that are identical when read from the 5'to 3'direction on one strand and the 3'to 5' direction on the other strand are

Accepted Answer

A)  palindromes.
B)  reverse transcriptases.
C)  restriction endonucleases.
D)  ligases.
E)  DNA polymerases.F) All of the above
G) A) and C)

Question 7

The deliberate removal of genetic material from one organism and combining it with the genetic material of another organism is a specific technique called

Accepted Answer

A)  genetic engineering.
B)  biotechnology.
C)  recombinant DNA technology.
D)  gel electrophoresis.
E)  gene probes.F) A) and D)
G) C) and E)

Question 8

The human gene for a protein hormone has been isolated.Scientists want to introduce this gene into a bacterial host, modifying the bacteria so that it can be used to produce this hormone.After successfully introducing the gene on a plasmid, the bacteria produced the protein hormone, but the protein was defective, longer than normal, with intervening stretches of amino acids not found in the native protein.What is the most likely explanation?

Accepted Answer

A)  The bacteria can't transcribe the human gene.
B)  The gene that was introduced contained introns.
C)  Bacterial cells cannot translate mRNAs transcribed from human genes.
D)  Bacterial ribosomes make errors when transcribing human mRNAs.E) A) and B)
F) All of the above

Question 9

Which of the following regions of DNA would be of most value for forensic analysis?

Accepted Answer

A)  DNA sequences that are typically identical for all humans
B)  DNA sequences that differ among humans, with a maximum of two different alleles
C)  DNA sequences that are quite variable with multiple different alleles
D)  Most DNA sequences are equally valuable for forensic analysis.E) B) and C)
F) All of the above C

Question 10

The human gene for a protein hormone has been isolated.Scientists want to introduce this gene into a bacterial host, modifying the bacteria so that it can be used to produce this hormone.After successfully introducing the gene on a plasmid, the bacteria produced the protein hormone, but the protein was defective, longer than normal, with intervening stretches of amino acids not found in the native protein.Which solution would be most likely to resolve this problem?

Accepted Answer

A)  Treat the defective protein with enzymes.
B)  Introduce human ribosomes into the bacteria.
C)  Start over, introducing a complementary DNA (cDN
A)   copy of the gene into the bacterial host.
D)  Introduce human mRNAs into the bacterial host.E) A) and C)
F) A) and B)

Question 11

DNA strands can be clipped crosswise at selected positions by using enzymes called

Accepted Answer

A)  palindromes.
B)  reverse transcriptases.
C)  restriction endonucleases.
D)  ligases.
E)  DNA polymerases.F) D) and E)
G) B) and E)

Question 12

Reverse transcriptase is used to make cDNA from an RNA template.

Accepted Answer

A) True 
 B)False

Question 13

Which of the following is less subject to degradation than is chromosomal DNA and is used as an evolutionary time clock?

Accepted Answer

A)  mitochondrial DNA
B)  chloroplast DNA
C)  rRNA
D)  mRNA
E)  tRNAF) B) and E)
G) None of the above

Question 14

Which of the following is true of the analysis of DNA fragments using gel electrophoresis?

Accepted Answer

A)  Larger DNA fragments move slowly and remain closer to the wells.
B)  DNA has an overall negative charge and moves to the positive pole.
C)  DNA fragments are stained to visualize them.
D)  An electric current through the gel causes DNA fragments to migrate.
E)  All of these choices are correct.F) B) and C)
G) A) and E)

Question 15

In order to ensure compatibility between the target DNA and the plasmid DNA,

Accepted Answer

A)  target DNA is removed from cells and isolated.
B)  cloning host is treated with calcium chloride and receives plasmid.
C)  target DNA and plasmid are treated with the same restriction endonuclease.
D)  desired protein is produced by cloning host.
E)  gene is amplified by multiplication of cloning host.F) A) and E)
G) A) and D)

Question 16

Restriction endonucleases recognize and clip at DNA base sequences called _____.

Accepted Answer

The answer of Restriction endonucleases recognize and clip at DNA...

Question 17

The Western Blot technique detects

Accepted Answer

A)  DNA.
B)  RNA.
C)  proteins.
D)  recombinant DNA.
E)  specific genetic marker sequence on genes.F) A) and E)
G) C) and D)

Question 18

When determining the sequence of nucleotides in an unknown sample of DNA, which method is used to sequence the DNA?

Accepted Answer

A)  PCR
B)  Cloning
C)  The Sanger Method
D)  Southern blot hybridization
E)  Microarray analysisF) A) and B)
G) A) and C)

Question 19

Which of the following is a serious concern when performing PCR?

Accepted Answer

A)  The high temperature needed may denature the DNA.
B)  Restriction enzymes are difficult to obtain in adequate quantities.
C)  Contaminating DNA may be introduced and amplified.
D)  Lab personnel may be exposed to radiation.
E)  PCR is a very time-consuming process.F) A) and E)
G) A) and D) C

Question 20

Amplification of DNA is accomplished by

Accepted Answer

A)  polymerase chain reaction.
B)  DNA sequencing.
C)  gene probes.
D)  Southern blot.
E)  All of these choices are correct.F) B) and C)
G) C) and E) A

Exam 10: Genetic Engineering: a Revolution in Molecular Biology

Sequencing a gene automatically identifies which gene it is.

Correct Answer
verified

The process of introducing a needed normal gene into human cells is called DNA mapping.

Correct Answer
verified

Which PCR step causes the denaturation of double-stranded DNA?

Correct Answer
verified

An example of gene therapy is the insertion of the gene for human growth hormone into E.coli cells.

Correct Answer
verified

The function of the dideoxy (dd) nucleotides that are used in the Sanger method of DNA sequencing is to

Correct Answer
verified

Sequences of DNA that are identical when read from the 5'to 3'direction on one strand and the 3'to 5' direction on the other strand are

Correct Answer
verified

The deliberate removal of genetic material from one organism and combining it with the genetic material of another organism is a specific technique called

Correct Answer
verified

Correct Answer
verified

Which of the following regions of DNA would be of most value for forensic analysis?

Correct Answer
verified
C

Correct Answer
verified

DNA strands can be clipped crosswise at selected positions by using enzymes called

Correct Answer
verified

Reverse transcriptase is used to make cDNA from an RNA template.

Correct Answer
verified

Which of the following is less subject to degradation than is chromosomal DNA and is used as an evolutionary time clock?

Correct Answer
verified

Which of the following is true of the analysis of DNA fragments using gel electrophoresis?

Correct Answer
verified

In order to ensure compatibility between the target DNA and the plasmid DNA,

Correct Answer
verified

Restriction endonucleases recognize and clip at DNA base sequences called _____.

Correct Answer
verified

The Western Blot technique detects

Correct Answer
verified

When determining the sequence of nucleotides in an unknown sample of DNA, which method is used to sequence the DNA?

Correct Answer
verified

Which of the following is a serious concern when performing PCR?

Correct Answer
verified
C

Amplification of DNA is accomplished by

Correct Answer
verified
A

Exam 10: Genetic Engineering: a Revolution in Molecular Biology

Sequencing a gene automatically identifies which gene it is.

Correct AnswerverifiedShow Answer

The process of introducing a needed normal gene into human cells is called DNA mapping.

Correct AnswerverifiedShow Answer

Which PCR step causes the denaturation of double-stranded DNA?

Correct AnswerverifiedShow Answer

An example of gene therapy is the insertion of the gene for human growth hormone into E.coli cells.

Correct AnswerverifiedShow Answer

The function of the dideoxy (dd) nucleotides that are used in the Sanger method of DNA sequencing is to

Correct AnswerverifiedShow Answer

Sequences of DNA that are identical when read from the 5'to 3'direction on one strand and the 3'to 5' direction on the other strand are

Correct AnswerverifiedShow Answer

The deliberate removal of genetic material from one organism and combining it with the genetic material of another organism is a specific technique called

Correct AnswerverifiedShow Answer

Correct AnswerverifiedShow Answer

Which of the following regions of DNA would be of most value for forensic analysis?

Correct AnswerverifiedC

Correct AnswerverifiedShow Answer

DNA strands can be clipped crosswise at selected positions by using enzymes called

Correct AnswerverifiedShow Answer

Reverse transcriptase is used to make cDNA from an RNA template.

Correct AnswerverifiedShow Answer

Which of the following is less subject to degradation than is chromosomal DNA and is used as an evolutionary time clock?

Correct AnswerverifiedShow Answer

Which of the following is true of the analysis of DNA fragments using gel electrophoresis?

Correct AnswerverifiedShow Answer

In order to ensure compatibility between the target DNA and the plasmid DNA,

Correct AnswerverifiedShow Answer

Restriction endonucleases recognize and clip at DNA base sequences called _____.

Correct AnswerverifiedShow Answer

The Western Blot technique detects

Correct AnswerverifiedShow Answer

When determining the sequence of nucleotides in an unknown sample of DNA, which method is used to sequence the DNA?

Correct AnswerverifiedShow Answer

Which of the following is a serious concern when performing PCR?

Correct AnswerverifiedC

Amplification of DNA is accomplished by

Correct AnswerverifiedA

Correct Answer
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Correct Answer
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C

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C

Correct Answer
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A