Question 1

Organisms that require increased pressure for optimum growth are called

Accepted Answer

A)  barotolerant.
B)  osmotolerant.
C)  barophilic.
D)  osmophilic.E) B) and D)
F) B) and C)

Question 2

The limitation on microbial growth rate at high nutrient levels is/are

Accepted Answer

A)  the rate of protein synthesis.
B)  the rate of DNA replication.
C)  the saturation of the transport proteins for nutrient uptake.
D)  All of the choices are correct.E) B) and C)
F) A) and D)

Question 3

Bacteria reproductive strategies include all of the following EXCEPT

Accepted Answer

A)  binary fission.
B)  budding.
C)  multiple fission.
D)  formation of mutinucleoid filaments that break apart into uninucleate spores.
E)  fusion of two bacterial cells that then split into four uninucleate cells.F) A) and E)
G) B) and C)

Question 4

At 4:00 p.m.a closed flask of sterile broth is inoculated with 10,000 cells.The lag phase lasts 1 hour.At 9:00 p.m.the culture enters stationary phase with a population of 65 million cells.At what time is the population half maximal?

Accepted Answer

A)  6:30 p.m.
B)  7:20 p.m.
C)  8:20 p.m.
D)  8:40 p.m.E) B) and C)
F) None of the above

Question 5

A culture in a closed vessel to which no additional medium is added and from which no waste products are removed is called a __________ culture.

Accepted Answer

A)  continuous
B)  batch
C)  fed-batch
D)  semicontinuousE) B) and D)
F) A) and B)

Question 6

How is the cell cycle of Sulfolobus spp.similar to that of eukaryotes?

Accepted Answer

A)  Sulfolobus has microtubules composed of a tubulin homolog that separate the chromosomes.
B)  Sulfolobus demonstrates prophase,metaphase,anaphase,and telophase.
C)  A centrosome is apparent in Sulfolobus during G2.
D)  The cytokinesis process in Sulfolobus is similar to the process in eukaryotes.E) B) and D)
F) B) and C)

Question 7

Quorum sensing

Accepted Answer

A)  is a phenomenon in which bacteria monitor their own population density.
B)  depends on the sensing of signal protein molecules.
C)  plays an important role in formation of biofilms by Pseudomonas aeruginosa.
D)  All of the choices are correct.E) A) and B)
F) None of the above

Question 8

What method was used to inoculate the plate shown here?
CDC
A.Pour plate
B.Spread plate
C.Streak plate
D.Swab plate

Accepted Answer

The answer of What method was used to inoculate the...

Question 9

The actin-like protein that seems to be involved in determining cell shape is

Accepted Answer

A)  MreB.
B)  ActA.
C)  SpnC.
D)  FtsZ.E) All of the above
F) A) and D)

Question 10

Most bacterial and archaeal cells divide by

Accepted Answer

A)  mitosis.
B)  meiosis.
C)  binary fission.
D)  All of the choices are correct.E) A) and D)
F) B) and C)

Question 11

Most microorganisms maintain their internal pH

Accepted Answer

A)  near neutral (pH 7) .
B)  near their optimum growth pH.
C)  slightly acidic (pH 4-6) .
D)  slightly alkaline (pH 8-10) .E) All of the above
F) C) and D)

Question 12

Membranes of some thermophilic Archaea are stabilized by ether linked phospholipids.

Accepted Answer

A) True 
 B)False

Question 13

There doesn't appear to be much diversity in the cell cycle processes of the Archaea,and Sulfolobus serves as an excellent model for all other Archaea.

Accepted Answer

A) True 
 B)False

Question 14

Which of the following is considered a cardinal growth temperature?

Accepted Answer

A)  The minimum temperature
B)  The maximum temperature
C)  The optimum temperature
D)  All of these are considered cardinal growth temperatures.E) B) and C)
F) A) and C)

Question 15

One hypothesis about the decline in cell numbers during death phase is that the cells aren't all dying but instead remain alive but unable to grow when cultured,at least temporarily.These are known as

Accepted Answer

A)  viable but nonculturable cells.
B)  ghost cells.
C)  death phase cells.
D)  programmed death cells.E) A) and C)
F) C) and D)

Question 16

Which of the following methods could be used to detect the presence of viable but nonculturable cells of a pathogen in a water or food specimen?

Accepted Answer

A)  Culture on enriched agar
B)  Use of enrichment techniques
C)  Polymerase chain reaction
D)  Culture on selective agarE) B) and C)
F) All of the above

Question 17

Organisms that grow in the mud under relatively nonturbulent bodies of water are likely to be

Accepted Answer

A)  obligate aerobes.
B)  thermophilic.
C)  barophilic.
D)  anaerobes.E) C) and D)
F) A) and C)

Question 18

A microbiologist is attempting to determine the size of a microbial population and has employed several methods.She noticed that the direct count she obtained using a counting chamber and microscope was much higher than her estimate from doing a viable plate count.This discrepancy was encountered during multiple trials.What is the most likely reason for the discrepancy?

Accepted Answer

A)  She counted incorrectly.
B)  She did not correctly do the serial dilutions prior to the viable plate count.
C)  Many of the organisms viewed during the direct count may be dead.
D)  The medium and growth conditions used for the viable count will not support the growth of the organisms.
E)  The organisms were not readily cultured on the viable count,either because many were dead or because the medium and growth conditions used for the viable count will not support the growth of the microorganism.F) A) and B)
G) B) and C)

Question 19

Membranes of thermophilic bacteria are stabilized by phospholipids with

Accepted Answer

A)  fatty acids that tend to be more saturated.
B)  fatty acids that have lower molecular weights.
C)  fatty acids that have more double bonds.
D)  All of the choices are correct.E) A) and C)
F) C) and D)

Question 20

Which of the following can be used to isolate pure cultures of bacteria from mixtures?

Accepted Answer

A)  Spread plates
B)  Streak plates
C)  Pour plates
D)  All of the choices are correct.E) A) and B)
F) A) and C)

Exam 7: Microbial Growth

The limitation on microbial growth rate at high nutrient levels is/are

Correct Answer
verified

Most microorganisms maintain their internal pH

Correct Answer
verified

Which of the following can be used to isolate pure cultures of bacteria from mixtures?

Correct Answer
verified

There doesn't appear to be much diversity in the cell cycle processes of the Archaea,and Sulfolobus serves as an excellent model for all other Archaea.

Correct Answer
verified

Organisms that grow in the mud under relatively nonturbulent bodies of water are likely to be

Correct Answer
verified

Membranes of thermophilic bacteria are stabilized by phospholipids with

Correct Answer
verified

Quorum sensing

Correct Answer
verified

Which of the following is considered a cardinal growth temperature?

Correct Answer
verified

What method was used to inoculate the plate shown here? CDC A.Pour plate B.Spread plate C.Streak plate D.Swab plate

Correct Answer
verified

Membranes of some thermophilic Archaea are stabilized by ether linked phospholipids.

Correct Answer
verified

Correct Answer
verified

Bacteria reproductive strategies include all of the following EXCEPT

Correct Answer
verified

At 4:00 p.m.a closed flask of sterile broth is inoculated with 10,000 cells.The lag phase lasts 1 hour.At 9:00 p.m.the culture enters stationary phase with a population of 65 million cells.At what time is the population half maximal?

Correct Answer
verified

How is the cell cycle of Sulfolobus spp.similar to that of eukaryotes?

Correct Answer
verified

Which of the following methods could be used to detect the presence of viable but nonculturable cells of a pathogen in a water or food specimen?

Correct Answer
verified

Most bacterial and archaeal cells divide by

Correct Answer
verified

The actin-like protein that seems to be involved in determining cell shape is

Correct Answer
verified

Organisms that require increased pressure for optimum growth are called

Correct Answer
verified

A culture in a closed vessel to which no additional medium is added and from which no waste products are removed is called a __________ culture.

Correct Answer
verified

One hypothesis about the decline in cell numbers during death phase is that the cells aren't all dying but instead remain alive but unable to grow when cultured,at least temporarily.These are known as

Correct Answer
verified

Exam 7: Microbial Growth

The limitation on microbial growth rate at high nutrient levels is/are

Correct AnswerverifiedShow Answer

Most microorganisms maintain their internal pH

Correct AnswerverifiedShow Answer

Which of the following can be used to isolate pure cultures of bacteria from mixtures?

Correct AnswerverifiedShow Answer

There doesn't appear to be much diversity in the cell cycle processes of the Archaea,and Sulfolobus serves as an excellent model for all other Archaea.

Correct AnswerverifiedShow Answer

Organisms that grow in the mud under relatively nonturbulent bodies of water are likely to be

Correct AnswerverifiedShow Answer

Membranes of thermophilic bacteria are stabilized by phospholipids with

Correct AnswerverifiedShow Answer

Quorum sensing

Correct AnswerverifiedShow Answer

Which of the following is considered a cardinal growth temperature?

Correct AnswerverifiedShow Answer

What method was used to inoculate the plate shown here? CDC A.Pour plate B.Spread plate C.Streak plate D.Swab plate

Correct AnswerverifiedShow Answer

Membranes of some thermophilic Archaea are stabilized by ether linked phospholipids.

Correct AnswerverifiedShow Answer

Correct AnswerverifiedShow Answer

Bacteria reproductive strategies include all of the following EXCEPT

Correct AnswerverifiedShow Answer

At 4:00 p.m.a closed flask of sterile broth is inoculated with 10,000 cells.The lag phase lasts 1 hour.At 9:00 p.m.the culture enters stationary phase with a population of 65 million cells.At what time is the population half maximal?

Correct AnswerverifiedShow Answer

How is the cell cycle of Sulfolobus spp.similar to that of eukaryotes?

Correct AnswerverifiedShow Answer

Which of the following methods could be used to detect the presence of viable but nonculturable cells of a pathogen in a water or food specimen?

Correct AnswerverifiedShow Answer

Most bacterial and archaeal cells divide by

Correct AnswerverifiedShow Answer

The actin-like protein that seems to be involved in determining cell shape is

Correct AnswerverifiedShow Answer

Organisms that require increased pressure for optimum growth are called

Correct AnswerverifiedShow Answer

A culture in a closed vessel to which no additional medium is added and from which no waste products are removed is called a __________ culture.

Correct AnswerverifiedShow Answer

One hypothesis about the decline in cell numbers during death phase is that the cells aren't all dying but instead remain alive but unable to grow when cultured,at least temporarily.These are known as

Correct AnswerverifiedShow Answer

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