Question 1

DNA polymerases are unable to replicate what areas of the chromosome?

Accepted Answer

A)   centromeres
B)   3′ ends
C)   origins of replication
D)   5′ endsE) B) and D)
F) None of the above

Question 2

You are studying DNA replication in human cells. You discover a mutant cell line that can carry out DNA replication,but the final product contains both RNA and DNA fragments. What enzyme is likely to be missing in this cell line?

Accepted Answer

A)   flap endonuclease
B)   DNA ligase
C)   DNA polymerase I
D)   DNA helicaseE) A) and B)
F) A) and C)

Question 3

DNA helicase enzymes move in what direction along the DNA during DNA replication?

Accepted Answer

A)   5′ to 3′
B)   3′ to 5′
C)   They travel in both directions.
D)   They remain stationary.E) A) and B)
F) A) and C)

Question 4

Recombination between sister chromatids produces new combinations of alleles that may be beneficial to the species.

Accepted Answer

A) True 
 B)False

Question 5

What enzyme relaxes supercoiling ahead of the replication fork?

Accepted Answer

A)   DNA ligase
B)   DNA primase
C)   topoisomerase
D)   DNA polymerase I
E)   DNA polymerase IIIF) B) and C)
G) A) and B)

Question 6

After the action of the helicase,single-stranded binding proteins keep the parental DNA strands from reforming a double helix.

Accepted Answer

A) True 
 B)False

Question 7

The proofreading of the DNA occurs in the ________.

Accepted Answer

A)   5′ to 3′ direction
B)   3′ to 5′ direction
C)   both directionsD) A) and B)
E) A) and C)

Question 8

A heteroduplex is a region within a DNA double helix that contains base mismatches that can lead to gene conversion.

Accepted Answer

A) True 
 B)False

Question 9

Select the statements that are true regarding the initiation of DNA replication in bacteria.

Accepted Answer

A)   It involves a region of the DNA called oriC.
B)   Proteins bind to the DNA to begin separation of the strands.
C)   The strands are initially separated at GC-rich regions of DNA.
D)   Following initial separation, DNA helicase enzymes continue to separate the strands.E) A) and D)
F) All of the above

Question 10

Replication usually begins in GC-rich regions due to the presence of only 2 hydrogen bonds between the bases.

Accepted Answer

A) True 
 B)False

Question 11

DNA polymerases add new nucleotides in what direction?

Accepted Answer

A)   5′ to 3′
B)   3′ to 5′
C)   both directionsD) None of the above
E) All of the above

Question 12

Which enzyme is responsible for the majority of DNA synthesis during replication?

Accepted Answer

A)   DNA ligase
B)   DNA primase
C)   topoisomerase
D)   DNA polymerase I
E)   DNA polymerase IIIF) A) and B)
G) A) and D)

Question 13

Which of the following best describes the function of the RecA protein in E.coli?

Accepted Answer

A)   Promotes strand invasion and D-loop formation in the double-stranded break model
B)   Recognizes double-stranded breaks in the DNA
C)   Involved in the initial formation of Holliday junctions
D)   Resolves Holliday junctions into separate chromosomesE) A) and B)
F) All of the above

Question 14

Homologous recombination that leads to genetic diversity occurs during meiosis II.

Accepted Answer

A) True 
 B)False

Question 15

In the attachment of a nucleotide to a DNA strand,the cleavage of the dNTP into deoxyribonucleoside monophosphate and pyrophosphate is ________.

Accepted Answer

A)   exergonic
B)   endergonic
C)   energy neutralD) A) and B)
E) A) and C)

Question 16

DNA polymerase III has an error in replication once every 100 million nucleotides.

Accepted Answer

A) True 
 B)False

Question 17

The problem of replicating the 3′ ends of chromosomes is solved by using the enzyme telomerase.

Accepted Answer

A) True 
 B)False

Question 18

The movement of the replication fork in bacterial replication is unidirectional.

Accepted Answer

A) True 
 B)False

Question 19

DNA polymerase III carries out leading and lagging strand synthesis in prokaryotes. How many enzymes accomplish this task in eukaryotes (not counting primer creation) ?

Accepted Answer

A)   one
B)   two
C)   three
D)   fourE) A) and B)
F) A) and C)

Question 20

In E.coli,which protein(s) are necessary for the cell to recognize double-stranded breaks and conduct recombination during meiosis?

Accepted Answer

A)   RecG
B)   RecA
C)   RecBCD
D)   RuvABCE) A) and B)
F) A) and C)

Exam 13: Dna Replication and Recombination

After the action of the helicase,single-stranded binding proteins keep the parental DNA strands from reforming a double helix.

Correct AnswerverifiedShow Answer

DNA polymerases add new nucleotides in what direction?

Correct AnswerverifiedShow Answer

DNA polymerase III carries out leading and lagging strand synthesis in prokaryotes. How many enzymes accomplish this task in eukaryotes (not counting primer creation) ?

Correct AnswerverifiedShow Answer

Recombination between sister chromatids produces new combinations of alleles that may be beneficial to the species.

Correct AnswerverifiedShow Answer

Which enzyme is responsible for the majority of DNA synthesis during replication?

Correct AnswerverifiedShow Answer

Select the statements that are true regarding the initiation of DNA replication in bacteria.

Correct AnswerverifiedShow Answer

Homologous recombination that leads to genetic diversity occurs during meiosis II.

Correct AnswerverifiedShow Answer

The problem of replicating the 3′ ends of chromosomes is solved by using the enzyme telomerase.

Correct AnswerverifiedShow Answer

You are studying DNA replication in human cells. You discover a mutant cell line that can carry out DNA replication,but the final product contains both RNA and DNA fragments. What enzyme is likely to be missing in this cell line?

Correct AnswerverifiedShow Answer

The proofreading of the DNA occurs in the ________.

Correct AnswerverifiedShow Answer

In E.coli,which protein(s) are necessary for the cell to recognize double-stranded breaks and conduct recombination during meiosis?

Correct AnswerverifiedShow Answer

Which of the following best describes the function of the RecA protein in E.coli?

Correct AnswerverifiedShow Answer

Replication usually begins in GC-rich regions due to the presence of only 2 hydrogen bonds between the bases.

Correct AnswerverifiedShow Answer

DNA polymerases are unable to replicate what areas of the chromosome?

Correct AnswerverifiedShow Answer

DNA polymerase III has an error in replication once every 100 million nucleotides.

Correct AnswerverifiedShow Answer

The movement of the replication fork in bacterial replication is unidirectional.

Correct AnswerverifiedShow Answer

What enzyme relaxes supercoiling ahead of the replication fork?

Correct AnswerverifiedShow Answer

In the attachment of a nucleotide to a DNA strand,the cleavage of the dNTP into deoxyribonucleoside monophosphate and pyrophosphate is ________.

Correct AnswerverifiedShow Answer

DNA helicase enzymes move in what direction along the DNA during DNA replication?

Correct AnswerverifiedShow Answer

A heteroduplex is a region within a DNA double helix that contains base mismatches that can lead to gene conversion.

Correct AnswerverifiedShow Answer

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